Dental infection is being considered as one of the six most widespread non-communicable diseases throughout the world. Berberis aquifolium have shown positive antimicrobial, antifungal and anti-inflammatory actions in several microbial studies. So, this study was conducted to determine the antimicrobial efficacy of B. aquifolium against dental biofilm forming Streptococcus mutans and common oral pathogens.
The dental caries samples were collected from 5 patients of age group 7-35yrs from Kottayam Dist., Kerala, under aseptic condition. All the collected plate samples were cultured on nutrient, blood and Mitis Salivary Agar plates and colonies were counted. Microbial species were identified on the basis of morphological and biochemical studies. Microbiological assay (agar well diffusion method and minimal inhibitory concentration (MIC) to determine inhibition zone against oral pathogens were performed. Thus this study concluded that the ethanolic extracts of B. aquifolium showed significant MIC against S. mutans, Candida albicans and Pseudomonas aeruginosa compared to that of standard drug.
Contents
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Abstract
1. Introduction
1.1. Objective of the Study
2. Review of Literature
2.1 Teeth and its structure
2.2 Pathogenicity of dental infection
2.3 Dental caries
2.4 Microbial etiology of caries
2.5 Treatment of caries
3. Materials and Methods
3.1 Chemicals
3.2 Preparation of powdered form of Homeopathic mother tinctures (HMT)
3.3 Collection of dental caries samples
3.4 Isolation and screening of bacteria
3.5 Morphological and biochemical identification of isolates
3.6 Identification of Streptococcal Isolates
3.7 Physical characterization
3.8 Biochemical characterization
3.9 Anti-microbial screening of extracts
3.10 MIC determination
4. Results
4.1. Isolation of bacteria from plaque samples based on their colony morphology
4.2. Biochemical identification of Colony 2
4.3. Biochemical identification of Colony 8
4.4. Biochemical identification of Colony 9
4.5. Identification of Colony 1 (budding Yeast cells) by Germ tube assay
4.6. Screening of DMSO (Negative control) and standard drugs (Positive control)
4.7. Inhibitory effects of B. aquifolium against dental biofilm producers and normal dental
4.8. Minimum inhibitory concentration (MIC) of B. aquifolium against dental pathogens
5. Discussion
6. Summary and Conclusion
References
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- Dr RATHEESH MOHANAN (Author), Dr Nirmal Ghosh (Author), Sulumol Thomas (Author), Sheethal Sreevallabhan (Author), Svenia Jose (Author), Asha Sukumarapillai (Author), Parvathy UJ (Author), Dr Sandya Sukumaran (Author), Sony Rajan (Author), 2021, Antibacterial Effect of Berberis Aquifolium Homeopathic Mother Tincture against Biofilm Formers from Dental Plaque, Munich, GRIN Verlag, https://www.hausarbeiten.de/document/986994
